Analysis Note

Positive ControlHepG2 cells, Human Colon tissue, HeLa cells

Application

ELISA (see comments)Immunoblotting (1-5 g/ml)Immunocytochemistry (10 g/ml)Paraffin Sections (3 g/ml)

General description

Purified mouse monoclonal antibody. Recognizes the ~37 kDa AKR1B10 protein.

Anti-AKR1B10. Mpuse monoclonal, clone 1A6 recognizes the ~37 kDa AKR1B10 protein in HepG2 and HeLa cells and human colon tissue. It is validated for ELISA, WB, ICC, and paraffin sections.

Recognizes the ~37 kDa AKR1B10 protein in HepG2 and HeLa cells and human colon tissue.

Immunogen

A recombinant polypeptide corresponding to amino acids 76-144 of human AKR1B10, expressed as a GST fusion protein

NA

Legal Information

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

Other Notes

Ahn K.H., et al. 2010 JOURNAL OF HEALTH SCIENCE56, 287.Satow, R., et al. 2010. Clin. Cancer Res.16, 2518.

For ELISA, this antibody can be used as the capture antibody; recommended concentration is 10 ng/ml, 1 µg/well, 100 µl total volume. Variables associated with assay conditions will dictate the proper working dilution.

Physical form

In PBS, pH 7.2.

Reconstitution

Following initial thaw, aliquot and freeze (-20°C).

Warning

Toxicity: Regulatory Review (Z)